P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo
(Plasmid
#170321)
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PurposeExpresses HA and mCherry tagged RPL22, neomycin resistance
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170321 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRPL22
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SpeciesH. sapiens (human)
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MutationNone
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Entrez GeneRPL22 (a.k.a. EAP, HBP15, HBP15/L22, L22)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo was a gift from Hemali Phatnani (Addgene plasmid # 170321 ; http://n2t.net/addgene:170321 ; RRID:Addgene_170321) -
For your References section:
Cell Type-Specific In Vitro Gene Expression Profiling of Stem Cell-Derived Neural Models. Gregory JA, Hoelzli E, Abdelaal R, Braine C, Cuevas M, Halpern M, Barretto N, Schrode N, Akbalik G, Kang K, Cheng E, Bowles K, Lotz S, Goderie S, Karch CM, Temple S, Goate A, Brennand KJ, Phatnani H. Cells. 2020 Jun 5;9(6). pii: cells9061406. doi: 10.3390/cells9061406. 10.3390/cells9061406 PubMed 32516938