pACEBac1-Q22YU3 del C3-2xStrep
(Plasmid
#170316)
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PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACEBac1
- Total vector size (bp) 6273
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQ22YU3 C-terminal truncation (Delta-C3)
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SpeciesTetrahymena thermophila
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Insert Size (bp)3312
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MutationDeletion of C-terminal C3-finger
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GenBank IDTTHERM_00122270 XM_001010823.2
- Promoter polyhedrin
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Tag
/ Fusion Protein
- Strep-Tag II (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer polyH
- 3′ sequencing primer SV40 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACEBac1-Q22YU3 del C3-2xStrep was a gift from Andrew Carter (Addgene plasmid # 170316 ; http://n2t.net/addgene:170316 ; RRID:Addgene_170316) -
For your References section:
Shulin packages axonemal outer dynein arms for ciliary targeting. Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP. Science. 2021 Feb 26;371(6532):910-916. doi: 10.1126/science.abe0526. 10.1126/science.abe0526 PubMed 33632841