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pACEBac1-Q22YU3-2xStrep
(Plasmid #170315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Total vector size (bp) 6561
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Q22YU3
  • Species
    Tetrahymena thermophila
  • Insert Size (bp)
    3600
  • GenBank ID
    TTHERM_00122270 XM_001010823.2
  • Entrez Gene
    TTHERM_00122270 (a.k.a. TTHERM_00122270)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • Strep-Tag II (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC sequencing identified an A707V discrepancy relative to XP_001010823.2. The depositing laboratory has confirmed that this should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEBac1-Q22YU3-2xStrep was a gift from Andrew Carter (Addgene plasmid # 170315 ; http://n2t.net/addgene:170315 ; RRID:Addgene_170315)

  • For your References section:

    Shulin packages axonemal outer dynein arms for ciliary targeting. Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP. Science. 2021 Feb 26;371(6532):910-916. doi: 10.1126/science.abe0526. 10.1126/science.abe0526 PubMed 33632841
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