pBluescript-Q22YU3-KO
(Plasmid
#170313)
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PurposePlasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170313 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Total vector size (bp) 7185
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Vector typeTetrahymena somatic gene knockout vector
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Selectable markersParomomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameQ22YU3 knockout
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SpeciesTetrahymena thermophila
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Insert Size (bp)7185
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer actggccgtcgttttac
- 3′ sequencing primer gtcctttgtcgatactg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe original plasmids were received from Dr Kazufumi Mochizuki's laboratory
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Reference for source of plasmid backbone:
Kataoka, K., Schoeberl, U.E., and Mochizuki, K. (2010). Modules for C-terminal epitope tagging of Tetrahymena genes. J. Microbiol. Methods 82, 342–346.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBluescript-Q22YU3-KO was a gift from Andrew Carter (Addgene plasmid # 170313 ; http://n2t.net/addgene:170313 ; RRID:Addgene_170313) -
For your References section:
Shulin packages axonemal outer dynein arms for ciliary targeting. Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP. Science. 2021 Feb 26;371(6532):910-916. doi: 10.1126/science.abe0526. 10.1126/science.abe0526 PubMed 33632841