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pBluescript-Q22MS1-KO
(Plasmid #170312)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170312 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Total vector size (bp) 7245
  • Vector type
    Tetrahymena somatic gene knockout vector
  • Selectable markers
    Paromomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Q22MS1 knockout
  • Alt name
    -
  • Species
    Tetrahymena thermophila
  • Insert Size (bp)
    7245

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer actggccgtcgttttac
  • 3′ sequencing primer gtcctttgtcgatactg
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The original plasmids were received from Dr Kazufumi Mochizuki's laboratory.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for source of plasmid backbone:
Kataoka, K., Schoeberl, U.E., and Mochizuki, K. (2010). Modules for C-terminal epitope tagging of Tetrahymena genes. J. Microbiol. Methods 82, 342–346.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript-Q22MS1-KO was a gift from Andrew Carter (Addgene plasmid # 170312 ; http://n2t.net/addgene:170312 ; RRID:Addgene_170312)

  • For your References section:

    Shulin packages axonemal outer dynein arms for ciliary targeting. Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP. Science. 2021 Feb 26;371(6532):910-916. doi: 10.1126/science.abe0526. 10.1126/science.abe0526 PubMed 33632841
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