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Addgene

pCSIIbsr-4082NES(ForAMPK)
(Plasmid #170310)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170310 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSIIbsr
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    4082NES (FRET biosensor for AMPK activity)
  • Species
    Synthetic
  • Insert Size (bp)
    2300

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSIIbsr-4082NES(ForAMPK) was a gift from Michiyuki Matsuda (Addgene plasmid # 170310 ; http://n2t.net/addgene:170310 ; RRID:Addgene_170310)

  • For your References section:

    A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs. Konagaya Y, Terai K, Hirao Y, Takakura K, Imajo M, Kamioka Y, Sasaoka N, Kakizuka A, Sumiyama K, Asano T, Matsuda M. Cell Rep. 2017 Nov 28;21(9):2628-2638. doi: 10.1016/j.celrep.2017.10.113. 10.1016/j.celrep.2017.10.113 PubMed 29186696
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