Topo-Tbxas1_ISH_AS
(Plasmid
#170308)
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PurposeEncoding partial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-BluntII-TOPO
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 4000
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Vector typeFor IVF
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePartial anti-sense strand of the mouse Tbxas1 gene for in situ hybridization
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SpeciesM. musculus (mouse)
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Insert Size (bp)800
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GenBank ID
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Topo-Tbxas1_ISH_AS was a gift from Michiyuki Matsuda (Addgene plasmid # 170308 ; http://n2t.net/addgene:170308 ; RRID:Addgene_170308) -
For your References section:
Intravital imaging identifies the VEGF-TXA2 axis as a critical promoter of PGE2 secretion from tumor cells and immune evasion. Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M. Cancer Res. 2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 10.1158/0008-5472.CAN-20-4245. 10.1158/0008-5472.CAN-20-4245 PubMed 34035084