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LentiCRISPRv2hyg-Ptgs1
(Plasmid #170299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPRv2puro
  • Backbone manufacturer
    https://www.addgene.org/52961/
  • Modifications to backbone
    A puromycin-resistance gene in lentiCRISPR v2 vector was replaced with a hygromycin-resistance gene.
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A gRNA targeting the mouse Ptgs1 gene.
  • gRNA/shRNA sequence
    Ptgs1
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (unknown if destroyed)
  • 3′ cloning site BsmBI (unknown if destroyed)
  • 5′ sequencing primer GAGGGCCTATTTCCCAtgatt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiCRISPRv2hyg-Ptgs1 was a gift from Michiyuki Matsuda (Addgene plasmid # 170299 ; http://n2t.net/addgene:170299 ; RRID:Addgene_170299)

  • For your References section:

    Intravital imaging identifies the VEGF-TXA2 axis as a critical promoter of PGE2 secretion from tumor cells and immune evasion. Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M. Cancer Res. 2021 May 25. pii: 0008-5472.CAN-20-4245. doi: 10.1158/0008-5472.CAN-20-4245. 10.1158/0008-5472.CAN-20-4245 PubMed 34035084
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