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Addgene

LentiCRISPRv2bleo-Necl-5
(Plasmid #170294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPRv2puro
  • Backbone manufacturer
    https://www.addgene.org/52961/
  • Modifications to backbone
    A puromycin-resistant gene in lentiCRISPR v2 vector was replaced with a bleomycin-resistant gene.
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A gRNA targeting the mouse Necl-5 gene.
  • gRNA/shRNA sequence
    Necl-5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (unknown if destroyed)
  • 3′ cloning site BsmBI (unknown if destroyed)
  • 5′ sequencing primer hU6-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiCRISPRv2bleo-Necl-5 was a gift from Michiyuki Matsuda (Addgene plasmid # 170294 ; http://n2t.net/addgene:170294 ; RRID:Addgene_170294)

  • For your References section:

    Functional visualization of NK Cell-mediated killing of metastatic single tumor cells. Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M. Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269. 10.7554/eLife.76269 PubMed 35113018
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