pT2ADW-lox-mCherry-hyBRET-ERK-NLS
(Plasmid
#170290)
-
PurposeFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170290 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepT2ADW
- Backbone size w/o insert (bp) 6500
- Total vector size (bp) 11242
-
Modifications to backbonepT2ADW was constructed as follows: The D4Z4 insulator was inserted before the CAG promoter of pT2AL200R175 carrying Tol2 recombination sites. Then, the WPRE sequence of pCSII-EF was inserted before the poly A sequence.
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namelox-mCherry-lox-hyBRET-ERK-NLS
-
SpeciesSynthetic
-
Insert Size (bp)4000
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pT2ADW-lox-mCherry-hyBRET-ERK-NLS was a gift from Michiyuki Matsuda (Addgene plasmid # 170290 ; http://n2t.net/addgene:170290 ; RRID:Addgene_170290) -
For your References section:
Functional visualization of NK Cell-mediated killing of metastatic single tumor cells. Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M. Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269. 10.7554/eLife.76269 PubMed 35113018
