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XE92 XGCNF-EnR
(Plasmid #17029)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GCNF-CS2+ and pSP64T EnR
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XGCNF-EnR
  • Alt name
    gcnf
  • Alt name
    nr6a1
  • Alt name
    engrailed
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    en (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: Cut XGCNF with Bgl II, XhoI, fill and cip. Gel purify the 4.3 Kb fragment (= DNA binding domain of GCNF in CS2+).
Cut pSP64T EnR w/ R1 and BamHI and fill. Gel purify the 1.2 Kb fragment (= EnT domain plus 3' UTR of Beta globin).

Plasmid History:
Source: SP64T EnR is from Kevin (Kimelman lab), originally from Werton Lab.

Notes for Use:
-cut with Xba I and transcribe with SP6 for capped RNA.

Ref: Song et al., Developmental Biology, 1999.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE92 XGCNF-EnR was a gift from Randall Moon (Addgene plasmid # 17029 ; http://n2t.net/addgene:17029 ; RRID:Addgene_17029)

  • For your References section:

    A role for xGCNF in midbrain-hindbrain patterning in Xenopus laevis. Song K, Takemaru KI, Moon RT. Dev Biol. 1999 Sep 1;213(1):170-9. doi: 10.1006/dbio.1999.9368. 10.1006/dbio.1999.9368 PubMed 10452854
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