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pPBbsr2-Necl-5-ScNeo
(Plasmid #170283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Backbone size w/o insert (bp) 6800
  • Total vector size (bp) 11368
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein
  • Alt name
    Necl-5-ScNeo
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    2300
  • Entrez Gene
    Pvr (a.k.a. 3830421F03Rik, CD155, D7Ertd458e, HVED, PVS, Taa1, Tage4, mE4, necl-5)
  • Tag / Fusion Protein
    • mScarlet and mNeonGreen

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBbsr2-Necl-5-ScNeo was a gift from Michiyuki Matsuda (Addgene plasmid # 170283 ; http://n2t.net/addgene:170283 ; RRID:Addgene_170283)

  • For your References section:

    Functional visualization of NK Cell-mediated killing of metastatic single tumor cells. Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M. Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269. 10.7554/eLife.76269 PubMed 35113018
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