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Addgene

pETDuet-1 TIM8,13
(Plasmid #170282)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170282 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Total vector size (bp) 5900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yeast Tim8 and Tim13
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
    NM_001184382.1, NM_001181310.3
  • Entrez Gene
    TIM8 (a.k.a. YJR135W-A)
  • Tag / Fusion Protein
    • TEV-cleavable N-terminal His tag on Tim13 (on backbone) (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-1 TIM8,13 was a gift from Paul Schanda (Addgene plasmid # 170282 ; http://n2t.net/addgene:170282 ; RRID:Addgene_170282)

  • For your References section:

    Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sucec I, Wang Y, Dakhlaoui O, Weinhaupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P. Sci Adv. 2020 Dec 18;6(51). pii: 6/51/eabd0263. doi: 10.1126/sciadv.abd0263. Print 2020 Dec. 10.1126/sciadv.abd0263 PubMed 33355130
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