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Addgene

pET28a(+)-HisTim12_CtoA
(Plasmid #170281)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170281 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a+
  • Total vector size (bp) 5605
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yeast Tim12
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    mutated two non-conserved Cys to Ala
  • GenBank ID
    NM_001178439.1
  • Entrez Gene
    TIM12 (a.k.a. YBR091C, MRS5)
  • Tag / Fusion Protein
    • TEV-cleavable N-terminal His tag (on backbone) (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a(+)-HisTim12_CtoA was a gift from Paul Schanda (Addgene plasmid # 170281 ; http://n2t.net/addgene:170281 ; RRID:Addgene_170281)

  • For your References section:

    Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9.10.12. Weinhaupl K, Wang Y, Hessel A, Brennich M, Lindorff-Larsen K, Schanda P. Structure. 2021 May 3. pii: S0969-2126(21)00125-8. doi: 10.1016/j.str.2021.04.009. 10.1016/j.str.2021.04.009 PubMed 33974880
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