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Addgene

XE91 XGCNF-CS2+
(Plasmid #17028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XGCNF
  • Alt name
    gcnf
  • Alt name
    nr6a-1
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1333
  • GenBank ID
    U58683
  • Entrez Gene
    nr6a1 (a.k.a. gcnf, xEmGCNF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Stu I (unknown if destroyed)
  • 3′ cloning site Stu1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: XGCNF (accession number = U58683) PCR product.
OFNT: 70-1403 (1333 bp)

Notes for Use:
For making capped RNA (sense) cut with Not 1 and transcribe with SP6.
For in situ probe cut with Hind III and transcribe with T7.

Ref: Song et al., Developmental Biology, 1999.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE91 XGCNF-CS2+ was a gift from Randall Moon (Addgene plasmid # 17028 ; http://n2t.net/addgene:17028 ; RRID:Addgene_17028)

  • For your References section:

    A role for xGCNF in midbrain-hindbrain patterning in Xenopus laevis. Song K, Takemaru KI, Moon RT. Dev Biol. 1999 Sep 1;213(1):170-9. doi: 10.1006/dbio.1999.9368. 10.1006/dbio.1999.9368 PubMed 10452854
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