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Addgene

pGBK CUP1 Nxf1B
(Plasmid #170266)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170266 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Modified pGBKT7
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7002
  • Total vector size (bp) 8859
  • Modifications to backbone
    Replaced the ADH1 promoter with a full length modified CUP1 promoter (modified the BamHI and NdeI site).
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nuclear export factor 1, B6 allele
  • Alt name
    Nxf1B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1857
  • GenBank ID
    53319
  • Promoter CUP1
  • Tags / Fusion Proteins
    • GAL4 DNA Binding Domain (N terminal on backbone)
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBK CUP1 Nxf1B was a gift from Bruce Hamilton (Addgene plasmid # 170266 ; http://n2t.net/addgene:170266 ; RRID:Addgene_170266)

  • For your References section:

    Inducible Yeast Two-Hybrid with Quantitative Measures. Hernandez J, Ross K, Hamilton B. bioRxiv 10.1101/2021.07.01.450807
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