LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode
(Plasmid #170255)

• Purpose: Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
• Depositing Lab: Prashant Mali
• Publication: Parekh et al iScience. 2021 Sep 20;24(10):103149. doi: 10.1016/j.isci.2021.103149. eCollection 2021 Oct 22.

Backbone

• Vector backbone: Unknown
• Backbone size w/o insert (bp): 9514
• Vector type: Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SV40 Large T Antigen
• Species: Simian virus 40
• Insert Size (bp): 2124
• Promoter: EF1a

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: 5'-ttctcaagcctcagacagtgg-3'

Resource Information

• Supplemental Documents:
  • LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode.ape
• A portion of this plasmid was derived from a plasmid made by: Addgene #9053
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry