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LV_EF1a_MAPK9-P2A-Hygro_Barcode
(Plasmid #170238)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170238 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Backbone size w/o insert (bp) 9514
  • Vector type
    Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAPK9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1272
  • Entrez Gene
    MAPK9 (a.k.a. JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK)
  • Promoter EF1a
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sabatini and Wood Cancer Pathways ORFs Kit (#1000000072)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV_EF1a_MAPK9-P2A-Hygro_Barcode was a gift from Prashant Mali (Addgene plasmid # 170238 ; http://n2t.net/addgene:170238 ; RRID:Addgene_170238)

  • For your References section:

    Charting oncogenicity of genes and variants across lineages via multiplexed screens in teratomas. Parekh U, McDonald D, Dailamy A, Wu Y, Cordes T, Zhang K, Tipps A, Metallo C, Mali P. iScience. 2021 Sep 20;24(10):103149. doi: 10.1016/j.isci.2021.103149. eCollection 2021 Oct 22. 10.1016/j.isci.2021.103149 PubMed 34646987
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