LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode
(Plasmid #170222)

• Purpose: Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
• Depositing Lab: Prashant Mali
• Publication: Parekh et al iScience. 2021 Sep 20;24(10):103149. doi: 10.1016/j.isci.2021.103149. eCollection 2021 Oct 22.

Backbone

• Vector backbone: Unknown
• Backbone size w/o insert (bp): 9514
• Vector type: Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CDK4
• Species: H. sapiens (human)
• Insert Size (bp): 909
• Mutation: R24C
• Entrez Gene: CDK4 (a.k.a. CMM3, PSK-J3)
• Promoter: EF1a

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: 5'-ttctcaagcctcagacagtgg-3'

Resource Information

• Supplemental Documents:
  • LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode.ape
• A portion of this plasmid was derived from a plasmid made by: Addgene #82175

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry