pGBK CUP1 EGFP
(Plasmid #170210)

• Purpose: Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP
• Depositing Lab: Bruce Hamilton
• Publication: Hernandez et al bioRxiv

Backbone

• Vector backbone: Modified pGBKT7
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 7002
• Total vector size (bp): 7722
• Modifications to backbone: Replaced the ADH1 promoter with a full length modified CUP1 promoter (modified the BamHI and NdeI site).
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: enhanced green fluorescent protein
• Alt name: EGFP
• Species: Synthetic
• Insert Size (bp): 720
• Promoter: CUP1
• Tags / Fusion Proteins:
  • GAL4 DNA Binding Domain (N terminal on backbone)
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pGBK CUP1 EGFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry