R619-X67-527: His6-SARS-CoV-2 N
(Plasmid
#170204)
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PurposeE. coli expression of SARS-CoV-2 nucleocapsid protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest-527
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 N
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SpeciesSARS-CoV-2
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Entrez GeneN (a.k.a. GU280_gp10)
- Promoter T7
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA sequence optimized for E. coli expression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R619-X67-527: His6-SARS-CoV-2 N was a gift from Dominic Esposito (Addgene plasmid # 170204 ; http://n2t.net/addgene:170204 ; RRID:Addgene_170204)