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R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351
(Plasmid #170196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest-303
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 S (spike) B.1.351
  • Species
    SARS-CoV-2
  • Mutation
    L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484K, N501Y, D614G, A701V
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV51
  • Tag / Fusion Protein
    • 3C-His8-Strep2x2 (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DNA sequence optimized for mammalian expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351 was a gift from Dominic Esposito (Addgene plasmid # 170196 ; http://n2t.net/addgene:170196 ; RRID:Addgene_170196)

  • For your References section:

    Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays. Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W. Protein Expr Purif. 2020 Jun 4;174:105686. doi: 10.1016/j.pep.2020.105686. 10.1016/j.pep.2020.105686 PubMed 32504802
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