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XE40 pSP64T-PABP (Clone 63)
(Plasmid #17019)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Poly(A) binding protein
  • Alt name
    PABP
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2200
  • Entrez Gene
    pabpc1.S (a.k.a. XELAEV_18033915mg, pab1, pabp, pabp1, pabpc1, pabpc1-a, pabpc1-b, pabpc1.L, pabpc2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Bgl II (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: Poly(A) binding protein clone w/ Beta-globin NC regions made from clones E&F (Zelus et al, 1989), subcloned with Bgl II linkers.

Transcription: linearize with XbaI or SmaI and transcribe with SP6.

Plasmid History:
Constructed by Bruce Zelus.

Reference Zelus et al. 1989. Molec. Cell. Biol. 9:2756-2760.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE40 pSP64T-PABP (Clone 63) was a gift from Randall Moon (Addgene plasmid # 17019 ; http://n2t.net/addgene:17019 ; RRID:Addgene_17019)

  • For your References section:

    Expression of the poly(A)-binding protein during development of Xenopus laevis. Zelus BD, Giebelhaus DH, Eib DW, Kenner KA, Moon RT. Mol Cell Biol. 1989 Jun;9(6):2756-60. doi: 10.1128/mcb.9.6.2756-2760.1989. 10.1128/mcb.9.6.2756-2760.1989 PubMed 2761544
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