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Addgene

modRNAc0-Cas9-2A-GFP
(Plasmid #170181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modRNA cap0 plasmid
  • Vector type
    IVT template plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9-2A-GFP
  • Species
    Synthetic
  • Promoter T7 promoter

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer tatttaggtgacactatag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    modRNAc0-Cas9-2A-GFP was a gift from Xiaojun Lian (Addgene plasmid # 170181 ; http://n2t.net/addgene:170181 ; RRID:Addgene_170181)

  • For your References section:

    Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells. Haideri T, Howells A, Jiang Y, Yang J, Bao X, Lian XL. Cell Rep Methods. 2022 Sep 7;2(9):100290. doi: 10.1016/j.crmeth.2022.100290. eCollection 2022 Sep 19. 10.1016/j.crmeth.2022.100290 PubMed 36160051
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