pCAG-ChroME2f-mRuby2-ST
(Plasmid
#170160)
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PurposeCation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170160 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChroME2f-mRuby2-ST
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-ChroME2f-mRuby2-ST was a gift from Hillel Adesnik (Addgene plasmid # 170160 ; http://n2t.net/addgene:170160 ; RRID:Addgene_170160) -
For your References section:
High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks. Sridharan S, Gajowa MA, Ogando MB, Jagadisan UK, Abdeladim L, Sadahiro M, Bounds HA, Hendricks WD, Turney TS, Tayler I, Gopakumar K, Oldenburg IA, Brohawn SG, Adesnik H. Neuron. 2022 Jan 27. pii: S0896-6273(22)00008-3. doi: 10.1016/j.neuron.2022.01.008. 10.1016/j.neuron.2022.01.008 PubMed 35120626
