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XLone-Cas9-2A-GFP
(Plasmid #170159)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    XLone
  • Backbone size w/o insert (bp) 6423
  • Total vector size (bp) 10527
  • Vector type
    Mammalian Expression, Synthetic Biology ; PiggyBac
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    spCas9
  • Species
    Synthetic
  • Insert Size (bp)
    4103
  • Promoter TRE3G promoter
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XLone-Cas9-2A-GFP was a gift from Xiaojun Lian (Addgene plasmid # 170159 ; http://n2t.net/addgene:170159 ; RRID:Addgene_170159)

  • For your References section:

    Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2. Ream MW, Randolph LN, Jiang Y, Chang Y, Bao X, Lian XL. Stem Cell Reports. 2024 Mar 5:S2213-6711(24)00044-4. doi: 10.1016/j.stemcr.2024.02.006. 10.1016/j.stemcr.2024.02.006 PubMed 38518781
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