pFastBac-CHD7 (1-1547)
(Plasmid
#170144)
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PurposeExpresses residues 1-1547 of CHD7 in insect cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170144 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBac1
- Backbone size w/o insert (bp) 4776
- Total vector size (bp) 9441
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namechromodomain helicase DNA binding protein 7
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SpeciesH. sapiens (human)
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Insert Size (bp)4670
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MutationC-terminal truncation of residues 1548-2997
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GenBank IDNM_017780
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Entrez GeneCHD7 (a.k.a. CRG, HH5, IS3, KAL5)
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer FastBacF
- 3′ sequencing primer FastBacR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac-CHD7 (1-1547) was a gift from Robert Kingston (Addgene plasmid # 170144 ; http://n2t.net/addgene:170144 ; RRID:Addgene_170144) -
For your References section:
Chromatin remodeling by the CHD7 protein is impaired by mutations that cause human developmental disorders. Bouazoune K, Kingston RE. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 10.1073/pnas.1213825109. Epub 2012 Nov 7. 10.1073/pnas.1213825109 PubMed 23134727
