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pFastBac-CHD7 (K999R)
(Plasmid #170142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone size w/o insert (bp) 4776
  • Total vector size (bp) 13848
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    chromodomain helicase DNA binding protein 7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9075
  • Mutation
    K999R
  • GenBank ID
    NM_017780
  • Entrez Gene
    CHD7 (a.k.a. CRG, HH5, IS3, KAL5)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-CHD7 (K999R) was a gift from Robert Kingston (Addgene plasmid # 170142 ; http://n2t.net/addgene:170142 ; RRID:Addgene_170142)

  • For your References section:

    Chromatin remodeling by the CHD7 protein is impaired by mutations that cause human developmental disorders. Bouazoune K, Kingston RE. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 10.1073/pnas.1213825109. Epub 2012 Nov 7. 10.1073/pnas.1213825109 PubMed 23134727
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