pMAD7-crRNA-2
(Plasmid
#170135)
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Purpose(Empty Backbone) For crRNA expression in monocotyledons protoplasts
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170135 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC (BsaI blocked)
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAD7-crRNA-2 was a gift from Caixia Gao (Addgene plasmid # 170135 ; http://n2t.net/addgene:170135 ; RRID:Addgene_170135) -
For your References section:
Genome editing in plants with MAD7 nuclease. Lin Q, Zhu Z, Liu G, Sun C, Lin D, Xue C, Li S, Zhang D, Gao C, Wang Y, Qiu JL. J Genet Genomics. 2021 May 3. pii: S1673-8527(21)00095-3. doi: 10.1016/j.jgg.2021.04.003. 10.1016/j.jgg.2021.04.003 PubMed 34120856