Circular 200,100 (mPCSK9)
(Plasmid #170122)

• Purpose: AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters
• Depositing Lab: Prashant Mali
• Publication: Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.1038/s41587-021-01171-4. doi: 10.1038/s41587-021-01171-4.

Backbone

• Vector backbone: pZac2.1
• Backbone manufacturer: Addgene #78601
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 6000
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Circular 200,100 guide RNA
• gRNA/shRNA sequence: GCCATCAGTCGCCGGTCCCAAGCCCGGATAAAATGGGAGGGGGCGGGAAACCGCCTAACCATGCCGACTGATGGCAGAAAAAAAAAACTTCCTGAAAGACAAATACACCCTAAGGCCTGAGAACTGAGGCCTTGCAGCGAGCATCCTCAGGTTCTGGTACACTGGAGCAGCTGGCCAGAGCCAGCCACAGCTTAATGTCTGATGGCAGAAAGCCAAGCACAGGCCCCCTGACCGTGAAGGAGGTGCTTCCATACACCAGGAATGGGTACCCAGGGTGAGACACCAAAAAAAAAACTGCCATCAGTCGGCGTGGACTGTAGAACACTGCCAATGCCGGTCCCAAGCCCGGATAAAAGTGGAGGGTACAGTCCACGC
• Species: M. musculus (mouse)
• Entrez Gene: Pcsk9 (a.k.a. AI415265, AI747682, FH3, HCHOLA3, Narc1, PC9)
• Promoter: Human U6 and mouse U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GACTATCATATGCTTACCGT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry