Pdpy-30-miniCAFE
(Plasmid
#170115)
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PurposeDpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170115 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMiniCAFE
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Alt nameTruncated VPR-dCjCas9
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Alt nameTruncated VPR-dCjeCas9
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SpeciesSynthetic; Campylobacter jejuni
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MutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9
- Promoter Dpy-30
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pdpy-30-miniCAFE was a gift from Zhili Rong (Addgene plasmid # 170115 ; http://n2t.net/addgene:170115 ; RRID:Addgene_170115) -
For your References section:
MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174. 10.1093/nar/gkab174 PubMed 33751124
