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RRRAA-5-mut-pHG165c
(Plasmid #170113)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170113 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHG165c
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RRRAA-5-MUT
  • Species
    Synthetic
  • Mutation
    5A14L, W116Q, E178K, S184T, S237L

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert has 5 nonsynonomous mutations and several synonmous mutations. Besides these mutations, this plasmid is the same as RRRAA-PhG165c (Addgene #)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RRRAA-5-mut-pHG165c was a gift from Liskin Swint-Kruse (Addgene plasmid # 170113 ; http://n2t.net/addgene:170113 ; RRID:Addgene_170113)

  • For your References section:

    Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes. Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, Swint-Kruse L. Proteins. 2021 Aug 8. doi: 10.1002/prot.26206. 10.1002/prot.26206 PubMed 34369028
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