pEcCas8
(Plasmid
#170093)
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Purposeexpresses E. coli type I-E Cas8
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
- Backbone size w/o insert (bp) 5214
- Total vector size (bp) 6720
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameE. coli type I-E Cas8
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SpeciesE. coli
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Insert Size (bp)1506
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gagatctcgatcccgcga (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byU.S. Department of Energy Joint Genome Institute
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
If used in a publication, please acknowledge that the plasmid pEcCas8 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEcCas8 was a gift from Chase Beisel (Addgene plasmid # 170093 ; http://n2t.net/addgene:170093 ; RRID:Addgene_170093) -
For your References section:
A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes. Wimmer F, Englert F, Beisel CL. Methods Mol Biol. 2022;2433:391-411. doi: 10.1007/978-1-0716-1998-8_24. 10.1007/978-1-0716-1998-8_24 PubMed 34985758