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Addgene

pEcCas5
(Plasmid #170090)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170090 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone size w/o insert (bp) 5221
  • Total vector size (bp) 5893
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E. coli type I-E Cas5
  • Species
    E. coli
  • Insert Size (bp)
    672
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    U.S. Department of Energy Joint Genome Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

If used in a publication, please acknowledge that the plasmid pEcCas5 was generated by U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility. The associated work was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEcCas5 was a gift from Chase Beisel (Addgene plasmid # 170090 ; http://n2t.net/addgene:170090 ; RRID:Addgene_170090)

  • For your References section:

    A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes. Wimmer F, Englert F, Beisel CL. Methods Mol Biol. 2022;2433:391-411. doi: 10.1007/978-1-0716-1998-8_24. 10.1007/978-1-0716-1998-8_24 PubMed 34985758
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