lentiGuideFE-Puro
(Plasmid #170069)

• Purpose: Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
• Depositing Lab: Neville Sanjana
• Publication: Morris et al Science. 2023 May 19;380(6646):eadh7699. doi: 10.1126/science.adh7699. Epub 2023 May 19.

Backbone

• Vector backbone: Custom
• Backbone size w/o insert (bp): 8670
• Total vector size (bp): 8770
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9 guide RNA scaffold with the F+E scaffold modification
• Insert Size (bp): 100
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: hU6-fwd (5'-GACTATCATATGCTTACCGT-3')
• 3′ sequencing primer: hGata4-rev (5'-ATTGTGGATGAATACTGCC-3')

Resource Information

• Supplemental Documents:
  • lentiGuideFE-Puro.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.07.438882 for bioRxiv preprint.