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Addgene

lentiCRISPRi(v1)-Blast
(Plasmid #170067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUGW
  • Backbone size w/o insert (bp) 8800
  • Total vector size (bp) 13100
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KRAB-dCas9
  • Insert Size (bp)
    4300
  • Promoter EFS-NS
  • Tag / Fusion Protein
    • 2A and 2xHA tag

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer EFS-NS-fwd (5'-gagaaccgtatataagtgcagt-3')
  • 3′ sequencing primer blast-R (5'-gctctttcaatgagggtgga-3'​)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.07.438882 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiCRISPRi(v1)-Blast was a gift from Neville Sanjana (Addgene plasmid # 170067 ; http://n2t.net/addgene:170067 ; RRID:Addgene_170067)

  • For your References section:

    Discovery of target genes and pathways at GWAS loci by pooled single-cell CRISPR screens. Morris JA, Caragine C, Daniloski Z, Domingo J, Barry T, Lu L, Davis K, Ziosi M, Glinos DA, Hao S, Mimitou EP, Smibert P, Roeder K, Katsevich E, Lappalainen T, Sanjana NE. Science. 2023 May 19;380(6646):eadh7699. doi: 10.1126/science.adh7699. Epub 2023 May 19. 10.1126/science.adh7699 PubMed 37141313
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