XE25 Dominant Negative Activin Receptor
(Plasmid #17005)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pSP64T
• Backbone size w/o insert (bp): 3000
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DN Activin Receptor
• Alt name: activin receptor
• Species: X. laevis (frog)
• Mutation: Dominant negative
• Entrez Gene: xstk2 (a.k.a. MGC80923)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: Sac II, blunt ended fragment. Transcription: linearize with EcoR1 and transcribe with SP6.

Plasmid History:
Constructed by Ali Hemmati-Brivanlou on 2/14/91.

For expression in embryos. Note that this construct does not have any 5' untranslated sequences and has lost the Bam H1 sits from the BS polylinker. (other names delta-1XAR1)