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Addgene

pKB235.2
(Plasmid #170025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101*
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mNeonGreen
  • Species
    Branchiostoma lanceolatum
  • Promoter PvirK

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    CCL20 alpha-helix
  • Species
    H. sapiens (human), Synthetic; Escherichia coli
  • Promoter Ptac
  • Tags / Fusion Proteins
    • Lpp (N terminal on insert)
    • OmpA (N terminal on insert)
    • Tether (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Peptide display fusion protein sequence was obtained from Bryan Davies and was originally published in Tucker et al. 2018 (DOI:10.1016/j.cell.2017.12.009).

Tucker AT, Leonard SP, DuBois CD, Knauf GA, Cunningham AL, Wilke CO, Trent MS, Davies BW. Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries. Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4. PMID: 29307492; PMCID: PMC5786472. Please visit https://www.biorxiv.org/content/10.1101/2021.06.01.446581v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKB235.2 was a gift from Jeffrey Tabor (Addgene plasmid # 170025 ; http://n2t.net/addgene:170025 ; RRID:Addgene_170025)

  • For your References section:

    High-throughput discovery of peptide activators of a bacterial sensor kinase. Brink KR, Mu AM, Hoang KV, Groszman K, Gunn JS, Tabor JJ. bioRxiv 10.1101/2021.06.01.446581
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