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pET-17b_Unc104-KIF5B-HALO
(Plasmid #169996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-17b
  • Backbone manufacturer
    Novagene
  • Backbone size w/o insert (bp) 3306
  • Total vector size (bp) 5958
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kinesin-like protein Unc-104 fused with KIF5B
  • Alt name
    Unc-104 / KIF1A (M1 TO V403) fused with KIF5B (E294 to K466)
  • Species
    H. sapiens (human), C. elegans (nematode)
  • Insert Size (bp)
    2652
  • Promoter T7
  • Tag / Fusion Protein
    • HALO-tag, 6xHIS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer NdeI
  • 3′ sequencing primer XhoI destroyed in cloning
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-17b_Unc104-KIF5B-HALO was a gift from Ron Vale (Addgene plasmid # 169996 ; http://n2t.net/addgene:169996 ; RRID:Addgene_169996)

  • For your References section:

    A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging. Niekamp S, Stuurman N, Vale RD. Nat Methods. 2020 Apr;17(4):437-441. doi: 10.1038/s41592-020-0782-3. Epub 2020 Mar 16. 10.1038/s41592-020-0782-3 PubMed 32203385
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