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pET-17b_K490clm_E215C
(Plasmid #169995)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-17b
  • Backbone manufacturer
    Novagene
  • Backbone size w/o insert (bp) 3306
  • Total vector size (bp) 4803
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kinesin family member 5B, isoform CRA_a
  • Alt name
    KIF5B K490clm
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1497
  • Mutation
    C7S, C65A, C168A, C174S, E215C, C294A, C330S, C421A
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-17b_K490clm_E215C was a gift from Ron Vale (Addgene plasmid # 169995 ; http://n2t.net/addgene:169995 ; RRID:Addgene_169995)

  • For your References section:

    Nanometer-accuracy distance measurements between fluorophores at the single-molecule level. Niekamp S, Sung J, Huynh W, Bhabha G, Vale RD, Stuurman N. Proc Natl Acad Sci U S A. 2019 Mar 5;116(10):4275-4284. doi: 10.1073/pnas.1815826116. Epub 2019 Feb 15. 10.1073/pnas.1815826116 PubMed 30770448
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