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Addgene

XE19 XIHbox 6
(Plasmid #16999)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16999 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    X1Hbox 6
  • Alt name
    hbox
  • Alt name
    hox
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    700

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II, Bam H1 (destroyed during cloning)
  • 3′ cloning site Bgl II (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: XIHbox 6 cDNA obtained by PCR amplification of neurula stage cDNA subcloned into SP64T.
Transcription: linearize with Bam H1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE19 XIHbox 6 was a gift from Randall Moon (Addgene plasmid # 16999 ; http://n2t.net/addgene:16999 ; RRID:Addgene_16999)
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