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Addgene

XE18 Noggin-CSKA
(Plasmid #16998)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16998 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCSKA
  • Backbone size w/o insert (bp) 4120
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    noggin PCR clone
  • Alt name
    noggin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    665
  • Entrez Gene
    nog.L (a.k.a. XELAEV_18043801mg, Noggin1, nog, nog-A, nog1, noggin-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: noggin PCR clone (coding region). In reverse orientation compared to CSKA promoter.

Plasmid History:
Constructed by Jan Christian.

Isolated and characterize by W. Smith and R. Harland.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE18 Noggin-CSKA was a gift from Randall Moon (Addgene plasmid # 16998 ; http://n2t.net/addgene:16998 ; RRID:Addgene_16998)
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