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Addgene

XE17 Noggin
(Plasmid #16997)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    noggin
  • Species
    X. laevis (frog)
  • Entrez Gene
    nog.L (a.k.a. XELAEV_18043801mg, Noggin1, nog, nog-A, nog1, noggin-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Bgl II (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: noggin PCR clone (coding region) in SP64T.
Transcription: linearize with EcoR1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian.

Noggin isolated and chacterized by W. Smith and R. Harland.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE17 Noggin was a gift from Randall Moon (Addgene plasmid # 16997 ; http://n2t.net/addgene:16997 ; RRID:Addgene_16997)
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