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Addgene

pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A
(Plasmid #169955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 LIC 6A
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARD8
  • Species
    H. sapiens (human)
  • Mutation
    S297A, R394E
  • Entrez Gene
    CARD8 (a.k.a. CARDINAL, DACAR, DAKAR, NDPP, NDPP1, TUCAN)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A was a gift from Hao Wu (Addgene plasmid # 169955 ; http://n2t.net/addgene:169955 ; RRID:Addgene_169955)

  • For your References section:

    Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H. Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 10.1016/j.immuni.2021.04.024. Epub 2021 May 20. 10.1016/j.immuni.2021.04.024 PubMed 34019797
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