Skip to main content
Addgene

XE15 Xbhh-C
(Plasmid #16995)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16995 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pT7TS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xbhh-C
  • Alt name
    xbhh
  • Alt name
    bhh
  • Alt name
    banded hedgehog
  • Species
    X. laevis (frog)
  • Entrez Gene
    ihh (a.k.a. bhh, X-hh, bhh-a)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression ConstructXenopus Banded Hedgehog.
(a.k.a. Xbhh; hh1)

Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.

References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)

Subclone Xbhh-N in pT7Ts.
Encodes amino terminal product with autoproteolytic cleavage. Linearize with BamH1 and transcribe with T7.

Subclone Xbhh-dominant negative-C in pT7TS:
Encodes a leader sequence, a few amino acids amino terminal to the autoproteolytic site, and the carboxy-terminal domain. Linearize with BamH1 and transcribe with T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE15 Xbhh-C was a gift from Randall Moon (Addgene plasmid # 16995 ; http://n2t.net/addgene:16995 ; RRID:Addgene_16995)

  • For your References section:

    Patterning of the neural ectoderm of Xenopus laevis by the amino-terminal product of hedgehog autoproteolytic cleavage. Lai CJ, Ekker SC, Beachy PA, Moon RT. Development. 1995 Aug;121(8):2349-60. doi: 10.1242/dev.121.8.2349. 10.1242/dev.121.8.2349 PubMed 7671801
Related items: