pFastBac HTB DPP9S S730A
(Plasmid
#169946)
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PurposePurification of catalytically-dead DPP9S from insect cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169946 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBac HTB
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDPP9
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Alt nameDPP9S
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SpeciesH. sapiens (human)
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MutationS730A
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Entrez GeneDPP9 (a.k.a. DP9, DPLP9, DPP IX, DPRP-2, DPRP2, HATIS, IMD111)
- Promoter polyhedrin
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Tag
/ Fusion Protein
- HIS-TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer polyhedrin-fwd
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac HTB DPP9S S730A was a gift from Hao Wu (Addgene plasmid # 169946 ; http://n2t.net/addgene:169946 ; RRID:Addgene_169946) -
For your References section:
DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation. Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H. Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 10.1038/s41586-021-03350-4. 10.1038/s41586-021-03350-4 PubMed 33731932
