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Bison sgRNA Library
(Pooled Library #169942)

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  • Purpose

    The BISON CRISPR library targets 713 E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes.

  • Vector Backbone

    lentiGuide-Puro (Plasmid #52963) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 169942 gRNA pooled library† 1 $380 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lentiCas9-Blast (Addgene #52962) or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    713 E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes
  • gRNAs
    2,852
  • Controls
    10 essential genes & 10 non-essential genes
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR Metabolic Gene Knockout library was a gift from Benjamin Ebert (Addgene #169942)

  • For your References section:

    The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K. Słabicki M, Kozicka Z, Petzold G, Li Y, Manojkumar M, Bunker RD, Donovan KA, Sievers QL, Koeppel J, Suchyta D, Sperling AS, Fink EC, Gasser JA, Wang LR, Corsello SM, Sellar RS, Jan M, Gillingham D, Scholl C, Fröhling S, Golub TR, Fischer ES, Thomä NH, Ebert BL. Nature. 2020 Sep;585(7824):293-297. doi: 10.1038/s41586-020-2374-x. PubMed 32494016
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