BCL6-GFP G55A in Artichoke
(Plasmid
#169935)
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PurposeOverexpression of BCL6-GFP G55A mutant fusion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneArtichoke (Plasmid #73320)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBCL6 G55A
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SpeciesH. sapiens (human)
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Insert Size (bp)825
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Mutation160R>C
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Entrez GeneBCL6 (a.k.a. BCL5, BCL6A, LAZ3, ZBTB27, ZNF51)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BCL6-GFP G55A in Artichoke was a gift from Benjamin Ebert (Addgene plasmid # 169935 ; http://n2t.net/addgene:169935 ; RRID:Addgene_169935) -
For your References section:
Small-molecule-induced polymerization triggers degradation of BCL6. Slabicki M, Yoon H, Koeppel J, Nitsch L, Roy Burman SS, Di Genua C, Donovan KA, Sperling AS, Hunkeler M, Tsai JM, Sharma R, Guirguis A, Zou C, Chudasama P, Gasser JA, Miller PG, Scholl C, Frohling S, Nowak RP, Fischer ES, Ebert BL. Nature. 2020 Dec;588(7836):164-168. doi: 10.1038/s41586-020-2925-1. Epub 2020 Nov 18. 10.1038/s41586-020-2925-1 PubMed 33208943