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CycK-GFP (1-270AA) in Cilantro (Codon optimized)
(Plasmid #169930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Cilantro 2 Plasmid #74450
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCNK
  • Alt name
    Cyclin K
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    810
  • Entrez Gene
    CCNK (a.k.a. CPR4, IDDHDF)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CycK-GFP (1-270AA) in Cilantro (Codon optimized) was a gift from Benjamin Ebert (Addgene plasmid # 169930 ; http://n2t.net/addgene:169930 ; RRID:Addgene_169930)

  • For your References section:

    The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K. Slabicki M, Kozicka Z, Petzold G, Li YD, Manojkumar M, Bunker RD, Donovan KA, Sievers QL, Koeppel J, Suchyta D, Sperling AS, Fink EC, Gasser JA, Wang LR, Corsello SM, Sellar RS, Jan M, Gillingham D, Scholl C, Frohling S, Golub TR, Fischer ES, Thoma NH, Ebert BL. Nature. 2020 Sep;585(7824):293-297. doi: 10.1038/s41586-020-2374-x. Epub 2020 Jun 3. 10.1038/s41586-020-2374-x PubMed 32494016
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