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Addgene

U6-U57A
(Plasmid #169927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4783
  • Total vector size (bp) 1217
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6 U57A
  • Alt name
    pRS314-U6 U57A
  • Alt name
    pAAH1027
  • Species
    S. cerevisiae (budding yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-U57A was a gift from Aaron Hoskins (Addgene plasmid # 169927 ; http://n2t.net/addgene:169927 ; RRID:Addgene_169927)

  • For your References section:

    Saccharomyces cerevisiae Ecm2 Modulates the Catalytic Steps of pre-mRNA Splicing. van der Feltz C, Nikolai B, Schneider C, Paulson JC, Fu X, Hoskins AA. RNA. 2021 Feb 5. pii: rna.077727.120. doi: 10.1261/rna.077727.120. 10.1261/rna.077727.120 PubMed 33547186
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